Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

Sensors

Amir Heidari³  Yong-Jin Yoon¹  Woo-Tae Park²  Pei-Chen Su¹  Jianmin Miao¹  Julius Tsai Ming Lin³ 

[1] Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore; E-Mails:;Department of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul 139743, Korea; E-Mail:;Institute of Microelectronics, Agency for Science, Technology and Research (ASTAR), Singapore 117685, Singapore; E-Mails:

关键词: biosensor;    capacitive filled gap transducer;    biotin;    streptavidin;   

DOI  :  10.3390/s140304585

来源: mdpi

【 摘 要 】

Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10⁻⁷ M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.