Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers

Molecules

Gui-Zhong Xin¹  Yin-Ching Lam¹  Maitinuer Maiwulanjiang¹  Gallant K. L. Chan¹  Kevin Yue Zhu¹  Wai-Lun Tang¹  Tina Ting-Xia Dong¹  Zi-Qi Shi²  Ping Li² 

[1] Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China; E-Mails:;State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China; E-Mail:

关键词: Bulbus Fritillariae Cirrhosae;    RAPD;    SCAR;    herbal drug authentication;   

DOI  :  10.3390/molecules19033450

来源: mdpi

【 摘 要 】

Bulbus Fritillariae is the most commonly used antitussive herb in China. Eleven species of Fritillaria are recorded as Bulbus Fritillariae in the Chinese Pharmacopoeia. Bulbus Fritillariae Cirrhosae is a group of six Fritillaria species with higher efficiency and lower toxicity derived mainly from wild sources. Because of their higher market price, five other Fritillaria species are often sold deceptively as Bulbus Fritillariae Cirrhosae in the herbal market. To ensure the efficacy and safety of medicinal herbs, the authentication of botanical resources is the first step in quality control. Here, a DNA based identification method was developed to authenticate the commercial sources of Bulbus Fritillariae Cirrhosae. A putative DNA marker (0.65 kb) specific for Bulbus Fritillariae Cirrhosae was identified using the Random Amplified Polymorphic DNA (RAPD) technique. A DNA marker representing a Sequence Characterized Amplified Region (SCAR) was developed from a RAPD amplicon. The SCAR marker was successfully applied to differentiate Bulbus Fritillariae Cirrhosae from different species of Fritillaria. Additionally, the SCAR marker was also useful in identifying the commercial samples of Bulbus Fritillariae Cirrhosae. Our results indicated that the RAPD-SCAR method was rapid, accurate and applicable in identifying Bulbus Fritillariae Cirrhosae at the DNA level.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.