IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts

Viruses

Lisa I. Hoover¹ 

[1] Maryland Pathogen Research Institute, College Park, MD 20742, USA; E-Mail:

关键词: West Nile virus;    interferon;    human dermal fibroblast;    antiviral response;    intracellular innate immunity;   

DOI  :  10.3390/v6031424

来源: mdpi

【 摘 要 】

Although dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York (WNV-NY), and nonneuroinvasive, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HDFs). However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon (IFN)-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.