Epigallocatechin-3-O-(3-O-methyl)-gallate-induced Differentiation of Human Keratinocytes Involves Klotho-Mediated Regulation of Protein Kinase-cAMP Responsive Element-Binding Protein Signaling

International Journal of Molecular Sciences

Hyoung-June Kim¹  Huikyoung Chang¹  Seung Hun Han²  Min Seuk Lee³  Ji-Yong Jung¹  SoonAe An¹  Seok-Yun Baek¹  Jin Ho Lee³  John Hwan Lee¹  Tae Ryong Lee¹  Dong Wook Shin¹ 

[1] Amorepacific Corporation R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Korea; E-Mails:;Department of Biological Sciences, Sungkyunkwan University, 300, Cheoncheon-dong, Jangan-gu, Suwon 440-746, Korea; E-Mail:;Sulloccha Research Center, Jangwon Co., Ltd., Jeju 699-924, Korea; E-Mails:

关键词: (−)-epigallocatechin-3-O-gallate;    methylated EGCG;    klotho;    keratinocyte differentiation;   

DOI  :  10.3390/ijms15045749

来源: mdpi

【 摘 要 】

(−)-Epigallocatechin-3-O-gallate (EGCG) has long been known as a potent inducer of keratinocyte differentiation. Although its molecular mechanisms have been extensively studied, its actions on human skin remain to be elucidated. In this study, we demonstrated that methylated EGCG and EGCG increase the expression of klotho, and that klotho functions as a downstream target of EGCG and methylated EGCG in keratinocyte differentiation. We demonstrated that methylated EGCG3 and EGCG induce morphological changes in normal human epidermal keratinocytes (NHEKs) that are related to up-regulation of klotho expression. We also demonstrated that a klotho-induced keratinocyte differentiation marker in NHEKs is inhibited by H-89, a protein kinase (PKA) inhibitor. These results suggest that methylated EGCG and EGCG may function as inducers of keratinocyte differentiation via transcriptional regulation of the klotho protein.

【 授权许可】

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