HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli

Molecules

Renyi Yan¹  Yangyang Cao¹ 

[1] Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; E-Mails:

关键词: Oroxylum indicum;    flavonoid;    HPLC-DPPH;    antioxidant;    rapid screening;   

DOI  :  10.3390/molecules19044409

来源: mdpi

【 摘 要 】

Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.