Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae

Metabolites

Daniel Veyel¹  Alexander Erban¹  Ines Fehrle¹  Joachim Kopka¹ 

[1] Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany; E-Mails:

关键词: Chlamydomonas reinhardtii;    metabolic engineering;    biofuels;    metabolite profiling;    GC-MS;    systems biology;   

DOI  :  10.3390/metabo4020184

来源: mdpi

【 摘 要 】

The generation of efficient production strains is essential for the use of eukaryotic microalgae for biofuel production. Systems biology approaches including metabolite profiling on promising microalgal strains, will provide a better understanding of their metabolic networks, which is crucial for metabolic engineering efforts. Chlamydomonas reinhardtii represents a suited model system for this purpose. We give an overview to genetically amenable microalgal strains with the potential for biofuel production and provide a critical review of currently used protocols for metabolite profiling on Chlamydomonas. We provide our own experimental data to underpin the validity of the conclusions drawn.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.