International Journal of Molecular Sciences | |
Indian Hedgehog in Synovial Fluid Is a Novel Marker for Early Cartilage Lesions in Human Knee Joint | |
Congming Zhang1  Xiaochun Wei1  Chongwei Chen1  Kun Cao1  Yongping Li1  Qiang Jiao1  Juan Ding1  Jingming Zhou3  Braden C. Fleming3  Qian Chen3  Xianwen Shang2  | |
[1] Department of Orthopaedics, the Second Hospital of Shanxi Medical University, Shanxi Key Lab of Bone and Soft Tissue Injury Repair, Taiyuan 030001, China; E-Mails:;Department of Orthopaedics, Guiyang Medical University, No. 9 Beijing Road, Guiyang 550004, China; E-Mail:;Department of Orthopaedics, Warren Alpert Medical School of Brown University, CORO West, Suite 402A, 1 Hoppin Street, Providence, RI 02903, USA; E-Mails: | |
关键词: Ihh; enzyme-linked immunosorbent assay; early cartilage lesions; | |
DOI : 10.3390/ijms15057250 | |
来源: mdpi | |
【 摘 要 】
To determine whether there is a correlation between the concentration of Indian hedgehog (Ihh) in synovial fluid (SF) and the severity of cartilage damage in the human knee joints, the knee cartilages from patients were classified using the Outer-bridge scoring system and graded using the Modified Mankin score. Expression of Ihh in cartilage and SF samples were analyzed with immunohistochemistry (IHC), western blot, and enzyme-linked immunosorbent assay (ELISA). Furthermore, we detected and compared Ihh protein levels in rat and mice cartilages between normal control and surgery-induced osteoarthritis (OA) group by IHC and fluorescence molecular tomography
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland
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