Proteomic Study of Differential Protein Expression in Mouse Lung Tissues after Aerosolized Ricin Poisoning

International Journal of Molecular Sciences

Zhendong Guo¹  Chao Han²  Jiajun Du³  Siyan Zhao¹  Yingying Fu¹  Guanyu Zheng¹  Yucheng Sun¹  Yi Zhang¹  Wensen Liu¹  Jiayu Wan¹  Jun Qian¹ 

[1] Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China; E-Mails:;263 Clinical Department of General Hospital of Beijing Military Region, Beijing 101149, China; E-Mail:;Medical Information Center of Chinese PLA General Hospital, Beijing 100853, China; E-Mail:

关键词: ricin;    aerosol;    lung injury;    proteomics;   

DOI  :  10.3390/ijms15057281

来源: mdpi

【 摘 要 】

Ricin is one of the most poisonous natural toxins from plants and is classified as a Class B biological threat pathogen by the Centers for Disease Control and Prevention (CDC) of U.S.A. Ricin exposure can occur through oral or aerosol routes. Ricin poisoning has a rapid onset and a short incubation period. There is no effective treatment for ricin poisoning. In this study, an aerosolized ricin-exposed mouse model was developed and the pathology was investigated. The protein expression profile in the ricin-poisoned mouse lung tissue was analyzed using proteomic techniques to determine the proteins that were closely related to the toxicity of ricin. 2D gel electrophoresis, mass spectrometry and subsequent biological functional analysis revealed that six proteins including Apoa1 apolipoprotein, Ywhaz 14-3-3 protein, Prdx6 Uncharacterized Protein, Selenium-binding protein 1, HMGB1, and DPYL-2, were highly related to ricin poisoning.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland