Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in Côte d’Ivoire and Vietnam

Viruses

Se Hun Gu¹¹  Burton K. Lim⁷  Blaise Kadjo¹⁰  Satoru Arai³  Jeong-Ah Kim⁶  Violaine Nicolas⁴  Aude Lalis⁴  Christiane Denys⁴  Joseph A. Cook¹  Samuel R. Dominguez²  Kathryn V. Holmes²  Lela Urushadze⁵  Ketevan Sidamonidze⁵  Davit Putkaradze⁵  Ivan V. Kuzmin^9,11  Michael Y. Kosoy^8,11  Jin-Won Song⁶ 

[1] Department of Biology, Museum of Southwestern Biology, University of New Mexico, Albuquerque, NM 87131, USA; E-Mail:;Department of Pediatrics, School of Medicine, University of Colorado, Aurora, CO 80045, USA; E-Mails:;Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; E-Mail:;Departement Systematique et Evolution, UMR CNRS 7205, Muséum National d’Histoire Naturelle, Paris 75005, France; E-Mails:;National Center for Disease Control and Public Health, Tbilisi 0177, Georgia; E-Mails:;Department of Microbiology, College of Medicine, Korea University, Seoul 136-705, Korea; E-Mails:;Department of Natural History, Royal Ontario Museum, Toronto, ON M5S 2C6, Canada; E-Mail:;Division of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA; E-Mail:;Global Alliance for Rabies Control, Manhattan, KS 66502, USA; E-Mail:;Department of Biology, Université de Cocody, Abidjan 22, Côte d’Ivoire; E-Mail:;Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA; E-Mail:

关键词: hantavirus;    Chiroptera;    evolution;   

DOI  :  10.3390/v6051897

来源: mdpi

【 摘 要 】

The recent discovery of genetically distinct hantaviruses in multiple species of shrews and moles prompted a further exploration of their host diversification by analyzing frozen, ethanol-fixed and RNAlater^®-preserved archival tissues and fecal samples from 533 bats (representing seven families, 28 genera and 53 species in the order Chiroptera), captured in Asia, Africa and the Americas in 1981–2012, using RT-PCR. Hantavirus RNA was detected in Pomona roundleaf bats (Hipposideros pomona) (family Hipposideridae), captured in Vietnam in 1997 and 1999, and in banana pipistrelles (Neoromicia nanus) (family Vespertilionidae), captured in Côte d’Ivoire in 2011. Phylogenetic analysis, based on the full-length S- and partial M- and L-segment sequences using maximum likelihood and Bayesian methods, demonstrated that the newfound hantaviruses formed highly divergent lineages, comprising other recently recognized bat-borne hantaviruses in Sierra Leone and China. The detection of bat-associated hantaviruses opens a new era in hantavirology and provides insights into their evolutionary origins.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.