Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals

Viruses

Karla Cautivo³  Tony Schountz⁴  Mariana Acu༚-Retamar²  Marcela Ferrés¹ 

[1] Laboratorio de Infectología y Virología Molecular, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile; E-Mail:;Facultad de Cs Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile; E-Mail:;Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile; E-Mail:;Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA; E-Mail:

关键词: Andes virus;    Chile;    ELISA;    strip immunoblot assay;    hantavirus;   

DOI  :  10.3390/v6052028

来源: mdpi

【 摘 要 】

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.