Sensors | |
Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media | |
Miriam Barquero-Quirós1  Olga Domínguez-Renedo2  Maria Asunción Alonso-Lomillo2  | |
[1] University of Costa Rica, CELEQ, San Pedro de Montes de Oca, 11500-2060 San José, Costa Rica;Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain; E-Mails: | |
关键词: acetylcholinesterase; biosensor; aluminum; acetylthiocholine iodide; screen-printed electrodes; gold nanoparticles; | |
DOI : 10.3390/s140508203 | |
来源: mdpi | |
【 摘 要 】
A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water. The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties.
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190026329ZK.pdf | 690KB | download |