The Discovery of Potentially Selective Human Neuronal Nitric Oxide Synthase (nNOS) Inhibitors: A Combination of Pharmacophore Modelling, CoMFA, Virtual Screening and Molecular Docking Studies

International Journal of Molecular Sciences

Guanhong Xu¹  Yue Chen¹  Kun Shen¹  Xiuzhen Wang¹  Fei Li¹ 

[1] School of Pharmacy, Nanjing Medical University, Nanjing 210029, China; E-Mails:

关键词: neuronal nitric oxide synthase;    inhibitors;    pharmacophore;    virtual screening;    docking;   

DOI  :  10.3390/ijms15058553

来源: mdpi

【 摘 要 】

Neuronal nitric oxide synthase (nNOS) plays an important role in neurotransmission and smooth muscle relaxation. Selective inhibition of nNOS over its other isozymes is highly desirable for the treatment of neurodegenerative diseases to avoid undesirable effects. In this study, we present a workflow for the identification and prioritization of compounds as potentially selective human nNOS inhibitors. Three-dimensional pharmacophore models were constructed based on a set of known nNOS inhibitors. The pharmacophore models were evaluated by Pareto surface and CoMFA (Comparative Molecular Field Analysis) analyses. The best pharmacophore model, which included 7 pharmacophore features, was used as a search query in the SPECS database (SPECS^®, Delft, The Netherlands). The hit compounds were further filtered by scoring and docking. Ten hits were identified as potential selective nNOS inhibitors.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland