Refolding Technologies for Antibody Fragments

Antibodies

Tsutomu Arakawa¹ 

[1] Alliance Protein Laboratories, 6042 Cornerstone Court West, San Diego, CA 92121, USA

关键词: refolding;    dialysis;    dilution;    C12-L-Glu;    antibody;    fragments;   

DOI  :  10.3390/antib3020232

来源: mdpi

【 摘 要 】

Refolding is one of the production technologies for pharmaceutical grade antibody fragments. Detergents and denaturants are primarily used to solubilize the insoluble proteins. The solubilized and denatured proteins are refolded by reducing the concentration of the denaturants or detergents. Several refolding technologies have been used for antibody fragments, comprising dilution, dialysis, solid phase solvent exchange and size exclusion chromatography, as reviewed here. Aggregation suppressor or folding-assisting agents, including arginine hydrochloride, ionic liquids and detergents or denaturants at low concentrations, are included in the refolding solvent to enhance refolding yield.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.