Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

International Journal of Molecular Sciences

Feng Qiu¹  Jingyuan Cao¹  Qiudong Su¹  Yao Yi¹ 

[1] Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China;

关键词: multiplex TaqMan real-time PCR;    hepatitis A virus;    hepatitis E virus;    identification;    application;   

DOI  :  10.3390/ijms15069780

来源: mdpi

【 摘 要 】

Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.