Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide

Sensors

Camille De Souza¹  Samia Zrig¹  Dengjun Wang¹  Minh-Chau Pham¹ 

[1] University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France;

关键词: reduced graphene oxide;    microRNA;    porphyrin;    hybridization;    reagentless detection;   

DOI  :  10.3390/s140609984

来源: mdpi

【 摘 要 】

Metalated porphyrins have been described to bind nucleic acids. Additionally, cobalt porphyrins present catalytic properties towards oxygen reduction. In this work, a carboxylic acid-functionalized cobalt porphyrin was physisorbed on reduced graphene oxide, then immobilized on glassy carbon electrodes. The carboxylic groups were used to covalently graft amino-terminated oligonucleotide probes which are complementary to a short microRNA target. It was shown that the catalytic oxygen electroreduction on cobalt porphyrin increases upon hybridization of miRNA strand (“signal-on” response). Current changes are amplified compared to non-catalytic amperometric system. Apart from oxygen, no added reagent is necessary. A limit of detection in the sub-nanomolar range was reached. This approach has never been described in the literature.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.