Quorum Sensing Activity in Pandoraea pnomenusa RB38

Sensors

Robson Ee¹  Yan-Lue Lim¹  Lin-Xin Kin¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;

关键词: matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF);    mass spectrometry (MS);    cell-to cell communication;    triple quodruopole liquid chromatography mass spectrometry (LC-MS/MS);    N-octanoyl homoserine lactone (C8-HSL);   

DOI  :  10.3390/s140610177

来源: mdpi

【 摘 要 】

Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography–mass spectrometry analysis was subsequently used to characterize the N-acyl homoserine lactone production profile of P. pnomenusa strain RB38, which validated that this isolate produced N-octanoyl homoserine lactone as a quorum sensing molecule. This is the first report of the production of N-octanoyl homoserine lactone by P. pnomenusa strain RB38.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.