Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells

Cells

Laura R. Sadofsky¹  Koti T. Sreekrishna²  Yakang Lin²  Renee Schinaman²  Kate Gorka²  Yogita Mantri²  John Christian Haught²  Thomas G. Huggins²  Robert J. Isfort²  Charles C. Bascom² 

[1] Cardiovascular and Respiratory Studies, The University of Hull, HU6 7RX, UK; E-Mails:;The Procter & Gamble Company, Mason, OH 45040, USA; E-Mails:

关键词: TRPV1;    TRPA1;    TRPA1;    TRPV1 co-expression;   

DOI  :  10.3390/cells3020616

来源: mdpi

【 摘 要 】

Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.