Surveillance of C-Allocation in Microalgal Cells

Metabolites

Heiko Wagner¹  Anne Jungandreas¹  Andrea Fanesi¹ 

[1] Universität Leipzig, Institute of Biology–Plant Physiology, Johannisallee 23, 04103 Leipzig, Germany; E-Mails:

关键词: FTIR spectroscopy;    algae;    biomass;    composition;    metabolomics;    chemometrics;    Chlamydomonas;   

DOI  :  10.3390/metabo4020453

来源: mdpi

【 摘 要 】

When microalgae are exposed to changing environmental conditions, e.g., light-dark cycles or oscillations in nutrient availability (CO₂, nitrogen, phosphate or silicate) they respond with metabolic changes in the carbon allocation pattern. Short time regulations in the time range of few seconds to minutes can be mirrored best by mass spectroscopy based metabolomics. However, these snap shots do not reflect the alterations in the carbon flow to the cellular macromolecules like protein, carbohydrate or lipid. In this review it is shown how the combination of FTIR spectroscopy and Chla-in-vivo-fluorescence based electron transport rates can reveal changes in the metabolic flux rates of carbon during a shift of the environmental conditions. The review will demonstrate in which time range FTIR spectroscopy can deliver significant information and how FTIR spectroscopy data can synergistically support metabolome analysis by mass-spectroscopy.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.