Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi

Energies

Jorge A. Ferreira¹  Patrik R. Lennartsson² 

[1] Swedish Centre for Resource Recovery, University of Borås, Borås SE-50190, Sweden;

关键词: Ascomycetes;    biomass;    ethanol;    protein;    thin stillage;    Zygomycetes;   

DOI  :  10.3390/en7063872

来源: mdpi

【 摘 要 】

A starch-based ethanol facility producing 200,000 m³ ethanol/year also produces ca. 2 million m³ thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a Zygomycete and four Ascomycetes were successfully grown in thin stillage containing 9% solids. Cultivation with Neurospora intermedia led to the production of ca. 16 g·L⁻¹ biomass containing 56% (w/w) crude protein, a reduction of 34% of the total solids, and 5 g·L⁻¹ additional ethanol. In an industrial ethanol production process (200,000 m³ ethanol/year), this can potentially lead to the production of 11,000 m³ extra ethanol per year. Cultivation with Aspergillus oryzae resulted in 19 g·L⁻¹ biomass containing 48% (w/w) crude protein and the highest reduction of the thin stillage glycerol (54%) among the Ascomycetes. Cultivation with Rhizopus sp. produced up to 15 g·L⁻¹ biomass containing 55% (w/w) crude protein. The spent thin stillage had been reduced up to 85%, 68% and 21% regarding lactic acid, glycerol and total solids, respectively. Therefore, N. intermedia, in particular, has a high potential to improve the ethanol process via production of additional ethanol and high-quality biomass, which can be considered for animal feed applications such as for fish feed.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.