Array Formatting of the Heat-Transfer Method (HTM) for the Detection of Small Organic Molecules by Molecularly Imprinted Polymers

Sensors

Gideon Wackers¹  Thijs Vandenryt¹  Peter Cornelis¹  Evelien Kellens¹  Ronald Thoelen¹  Ward De Ceuninck¹  Patricia Losada-Pérez¹  Bart van Grinsven¹  Marloes Peeters¹ 

[1] Institute for Materials Research, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium; E-Mails:

关键词: heat-transfer method (HTM);    molecularly imprinted polymers (MIPs);    serotonin;    histamine;    l-nicotine;    array format;   

DOI  :  10.3390/s140611016

来源: mdpi

【 摘 要 】

In this work we present the first steps towards a molecularly imprinted polymer (MIP)-based biomimetic sensor array for the detection of small organic molecules via the heat-transfer method (HTM). HTM relies on the change in thermal resistance upon binding of the target molecule to the MIP-type receptor. A flow-through sensor cell was developed, which is segmented into four quadrants with a volume of 2.5 μL each, allowing four measurements to be done simultaneously on a single substrate. Verification measurements were conducted, in which all quadrants received a uniform treatment and all four channels exhibited a similar response. Subsequently, measurements were performed in quadrants, which were functionalized with different MIP particles. Each of these quadrants was exposed to the same buffer solution, spiked with different molecules, according to the MIP under analysis. With the flow cell design we could discriminate between similar small organic molecules and observed no significant cross-selectivity. Therefore, the MIP array sensor platform with HTM as a readout technique, has the potential to become a low-cost analysis tool for bioanalytical applications.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.