Targeted Lipid Analysis of Haemolytic Mycelial Extracts of Aspergillus niger

Molecules

Maruᘚ Novak²  Kristina Sepčić²  Nada Kraᘞvec³  Igor Kri៪j¹  Peter Maპk²  Gregor Anderluh³  Graziano Guella⁴ 

[1] Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, 1000 Ljubljana, Slovenia, E-Mail:;Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia; E-Mails:;National Institute of Chemistry, 1000 Ljubljana, Slovenia; E-Mails:;Department of Physics, Bioorganic Chemistry Laboratory, University of Trento, Via Sommarive 14, I-38123 Povo-Trento, Italy; E-Mail:

关键词: Aspergillus niger;    haemolytic activity;    fatty acids;    lipid composition;    liquid chromatography-selectrospray ionisation tandem mass spectrometry;   

DOI  :  10.3390/molecules19079051

来源: mdpi

【 摘 要 】

Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1–3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography–electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.