Exhaled Breath Condensate for Proteomic Biomarker Discovery

Chromatography

Sean W. Harshman²  Claude C. Grigsby¹ 

[1] Air Force Research Laboratory, 711th Human Performance Wing/RHXBC, Wright-Patterson AFB, OH 45433, USA; E-Mail:;UES Inc., Air Force Research Laboratory, 711th Human Performance Wing/RHXBC, Wright-Patterson AFB, OH 45433, USA

关键词: exhaled breath condensate;    proteomics;    mass spectrometry;   

DOI  :  10.3390/chromatography1030108

来源: mdpi

【 摘 要 】

Exhaled breath condensate (EBC) has been established as a potential source of respiratory biomarkers. Compared to the numerous small molecules identified, the protein content of EBC has remained relatively unstudied due to the methodological and technical difficulties surrounding EBC analysis. In this review, we discuss the proteins identified in EBC, by mass spectrometry, focusing on the significance of those proteins identified. We will also review the limitations surrounding mass spectral EBC protein analysis emphasizing recommendations to enhance EBC protein identifications by mass spectrometry. Finally, we will provide insight into the future directions of the EBC proteomics field.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.