Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s

Insects

Alexander M. Campbell¹  Andrew J. Lawrence²  Caleb B. Hudspath² 

[1] Penn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USA;

关键词: Diaspididae;    armored scale insects;    phylogenetics;    molecular barcoding;    invasive species;    cryptic species;   

DOI  :  10.3390/insects5030528

来源: mdpi

【 摘 要 】

Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.