Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum

Molecules

Chi-Yu He²  Jie Fu²  Jing-Yi Ma²  Ru Feng²  Xiang-Shan Tan²  Min Huang²  Jia-Wen Shou²  Zhen-Xiong Zhao²  Xiao-Yang Li²  Xian-Feng Zhang¹  Yangchao Chen³ 

[1] Department of Neurosurgery, First Hospital, Jilin University, Changchun 130021, China;State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China;School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China

关键词: Polygonum capitatum;    extracts;    intestinal bacteria;    metabolites;    LC/MSⁿ-IT-TOF;   

DOI  :  10.3390/molecules190710291

来源: mdpi

【 摘 要 】

Polygonum capitatum Buch.-Ham.ex D. Don, a traditional Miao-nationality herbal medicine, has been widely used in the treatment of various urologic disorders. Recent pharmacological studies demonstrated that a pure compound, FR429, isolated from the ethanol extracts of P. capitatum could selectively inhibit the growth of four hepatocellular carcinoma (HCC) cell lines in a dose-dependent manner. Thus, P. capitatum probably exhibits potential antitumor activity. However, there is very little information on the metabolism of substances present in P. capitatum extracts. In this study, gallic acid, quercetrin, ethanol extracts and ethyl acetate fraction of ethnolic extract (EtOAc fraction) of P. capitatum were cultured anaerobically with rat intestinal bacteria. A highly sensitive and selective liquid chromatography electrospray ionization-ion trap-time of fight mass spectrometry (LC/MSⁿ-IT-TOF) technique was employed to identify and characterize the resulting metabolites. A total of 22 metabolites (M1–M22), including tannins, phenolic acids and flavonoids, were detected and characterized. The overall results demonstrated that the intestinal bacteria played an important role in the metabolism of P. capitatum, and the main metabolic pathways were hydrolysis, reduction and oxidation reactions. Our results provided a basis for the estimation of the metabolic transformation of P. capitatum in vivo.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.