MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

Molecules

Lo໼ Becker²  Vincent Carré²  Anne Poutaraud¹  Didier Merdinoglu¹ 

[1] Institut National de Recherche en Agronomie (INRA) – Santé de la Vigne et Qualité du Vin (UMR 1131), 28 rue de Herrlisheim, F-68021 Colmar, France; E-Mails:;Laboratoire de Chimie et Physique-Approche Multi échelle des Milieux Complexes (LCP-A2MC), Institut Jean Barriol (FR 2843), Université de Lorraine, ICPM 1 Boulevard Arago, F-57078 Metz, France; E-Mails:

关键词: mass spectrometry imaging;    grapevine;    stilbene phytoalexin;    viniferin;    resveratrol;    pterostilbene;    MALDI;    Plasmopara viticola;    Vitis vinifera;   

DOI  :  10.3390/molecules190710587

来源: mdpi

【 摘 要 】

To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.

【 授权许可】

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.