Simultaneous Expression of Cancer Stem Cell-Like Properties and Cancer-Associated Fibroblast-Like Properties in a Primary Culture of Breast Cancer Cells

Cancers

Mami Ishikawa¹  Takahiro Inoue¹  Takuma Shirai¹  Kazuhiko Takamatsu¹  Shiori Kunihiro¹  Hirokazu Ishii¹ 

[1] Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan; E-Mails:

关键词: cancer-associated fibroblast;    cancer stem cell;    epithelial-mesenchymal transition;    HIF1A;    glycolysis;   

DOI  :  10.3390/cancers6031570

来源: mdpi

【 摘 要 】

The importance of cancer-associated fibroblasts (CAFs) in cancer biology has been recently highlighted owing to their critical roles in cancer growth, progression, metastasis, and therapeutic resistance. We have previously established a primary culture of breast cancer cells, which showed epithelial-mesenchymal transition and cancer stem cell-like properties. In this study, we found that the primary culture also showed CAF-like properties. For example, hypoxia inducible factor 1α (HIF1A) and its downstream genes, nuclear factor-kappa B2 (NF-κB2) and BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3), and many enzymes involved in glycolysis, such as GAPDH, LDH, PGAM1, and PKM2, were highly overexpressed in the primary culture. Moreover, media conditioned with the primary culture cells enhanced the growth of breast cancer cells. Similar to previous CAF studies, this enhancement suggested to be occurred through fibroblast growth factor signaling. This MCKH primary culture cell, which showed simultaneous expression of tumorigenic and CAF properties, offers a unique experimental system for studying the biology of CAFs.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.