Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber

Sensors

Linh Viet Nguyen²  Sara Giannetti¹  Stephen Warren-Smith²  Alan Cooper³  Stefano Selleri¹  Annamaria Cucinotta¹ 

[1] Information Engineering Department, University of Parma, Parma 43100, Italy; E-Mails:;Institute for Photonics and Advanced Sensing (IPAS) and The ARC Centre for Nanoscale Biophotonics, The University of Adelaide, Adelaide 5005, Australia; E-Mails:;Australian Centre for Ancient DNA (ACAD), The University of Adelaide, Adelaide 5005, Australia; E-Mail:

关键词: genotyping;    suspended core optical fiber;    molecular beacon;    multiplexing;    DNA detection;   

DOI  :  10.3390/s140814488

来源: mdpi

【 摘 要 】

We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.