Localization of MLH3 at the Centrosomes

International Journal of Molecular Sciences

Lennart M. Roesner³  Christian Mielke²  Silke Faehnrich³  Yvonne Merkhoffer³  Kurt E. J. Dittmar¹  Hans G. Drexler³ 

[1] Helmholtz Centre of Infection Research, Braunschweig 38124, Germany; E-Mail:;Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich-Heine-University, Medical School, Düsseldorf 40225, Germany; E-Mail:;Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany; E-Mails:

关键词: DNA mismatch repair (MMR);    MLH3;    centrosome;   

DOI  :  10.3390/ijms150813932

来源: mdpi

【 摘 要 】

Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.