Bioevaluation of Novel Anti-Biofilm Coatings Based on PVP/Fe3O4 Nanostructures and 2-((4-Ethylphenoxy)methyl)-N-(arylcarbamothioyl)benzamides

Molecules

Carmen Limban⁷  Alexandru Vasile Missir⁷  Alexandru Mihai Grumezescu⁴  Alexandra Elena Oprea⁴  Valentina Grumezescu⁴  Bogdan Ștefan Vasile⁴  Gabriel Socol¹  Roxana Tru↜ă³  Miron Teodor Caproiu²  Mariana Carmen Chifiriuc⁸  Bianca Gălă𡯪nu⁵  Marieta Costache⁵  Laurențiu Moru↜iag⁷  Grațiela Pîrcălျioru⁶ 

[1] National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Romania; E-Mail:;The Organic Chemistry Center of Romanian Academy “Costin C.D. Nenitescu” Bucharest, Splaiul Independentei, 202B, 77208 Bucharest, Romania; E-Mail:;S.C. Metav-CD S.A., 31Rosetti Str., 020015 Bucharest, Romania; E-Mail:;Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania; E-Mails:;Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania; E-Mails:;Conway Institute, University College Dublin, Dublin 4, Dublin, Ireland;Department of Pharmaceutical Chemistry, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania; E-Mails:;Department of Microbiology, Faculty of Biology, University of Bucharest, Research Institute of University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania; E-Mail:

关键词: benzamides;    thiourea derivatives;    core/shell nanostructure;    magnetite;    anti-biofilm;   

DOI  :  10.3390/molecules190812011

来源: mdpi

【 摘 要 】

Novel derivatives were prepared by reaction of aromatic amines with 2-(4-ethylphenoxymethyl)benzoyl isothiocyanate, affording the N-[2-(4-ethylphenoxymethyl)benzoyl]-N'-(substituted phenyl)thiourea. Structural elucidation of these compounds was performed by IR, NMR spectroscopy and elemental analysis. The new compounds were used in combination with Fe₃O₄ and polyvinylpyrrolidone (PVP) for the coating of medical surfaces. In our experiments, catheter pieces were coated by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The microbial adherence ability was investigated in 6 multi-well plates by using culture based methods. The obtained surfaces were also assessed for their cytotoxicity with respect to osteoblast cells, by using fluorescence microscopy and MTT assay. The prepared surfaces by advanced laser processing inhibited the adherence and biofilm development ability of Staphylococcus aureus and Pseudomonas aeruginosa tested strains while cytotoxic effects on the 3T3-E1 preosteoblasts embedded in layer shaped alginate hydrogels were not observed. These results suggest that the obtained medical surfaces, based on the novel thiourea derivatives and magnetic nanoparticles with a polymeric shell could represent a promising alternative for the development of new and effective anti-infective strategies.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.