Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers

Bioengineering

Omotunde Olubi²  Laurisa London²  Biswajit Sannigrahi²  Peri Nagappan¹  Michael Williams³ 

[1] Center for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA 30314, USA; E-Mail:;Center for Functional Nanoscale Materials and Department of Chemistry, Clark Atlanta University, Atlanta, GA 30314, USA; E-Mails:;Department of Physics, Clark Atlanta University, Atlanta, GA 30314, USA; E-Mail:

关键词: block copolymers;    biosensor;    ionic polymerization;    atom transfer radical polymerization;    fluorescence microscope;   

DOI  :  10.3390/bioengineering1030134

来源: mdpi

【 摘 要 】

Biofunctional block copolymers are becoming increasingly attractive materials as active components in biosensors and other nanoscale electronic devices. We have described two different classes of block copolymers with biofuctional properties. Biofunctionality for block copolymers is achieved through functionalization with appropriate biospecific ligands. We have synthesized block copolymers of electroactive poly(3-decylthiophene) and 2-hydroxyethyl methacrylate by atom transfer radical polymerization. The block copolymers were functionalized with the dinitrophenyl (DNP) groups, which are capable of binding to Immunoglobulin E (IgE) on cell surfaces. The block copolymers were shown to be redox active. Additionally, the triblock copolymer of α, ω-bi-biotin (poly(ethylene oxide)-b-poly (styrene)-b-poly(ethylene oxide)) was also synthesized to study their capacity to bind fluorescently tagged avidin. The surface-active property of the poly(ethylene oxide) block improved the availability of the biotin functional groups on the polymer surfaces. Fluorescence microscopy observations confirm the specific binding of biotin with avidin.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.