Schisandrin B Induces Apoptosis and Cell Cycle Arrest of Gallbladder Cancer Cells

Molecules

Shan-Shan Xiang²  Xu-An Wang²  Huai-Feng Li²  Yi-Jun Shu²  Run-Fa Bao²  Fei Zhang²  Yang Cao²  Yuan-Yuan Ye²  Hao Weng²  Wen-Guang Wu²  Jia-Sheng Mu²  Xiang-Song Wu²  Mao-Lan Li²  Yun-Ping Hu²  Lin Jiang²  Zhu-Jun Tan²  Wei Lu²  Feng Liu¹ 

[1] The First Affiliated Hospital Nanchang University Emergency Unit, No. 17 Yongwai Road, Nanchang 330006, China;Department of General Surgery, School of Medicine, Shanghai Jiao Tong University, No. 1665 Kongjiang Road, Shanghai 200092, China

关键词: schisandrin B;    gallbladder cancer;    apoptosis;    cell cycle arrest;    mitochondrial pathway;   

DOI  :  10.3390/molecules190913235

来源: mdpi

【 摘 要 】

Gallbladder cancer, with high aggressivity and extremely poor prognosis, is the most common malignancy of the bile duct. The main objective of the paper was to investigate the effects of schisandrin B (Sch B) on gallbladder cancer cells and identify the mechanisms underlying its potential anticancer effects. We showed that Sch B inhibited the viability and proliferation of human gallbladder cancer cells in a dose-, time -dependent manner through MTT and colony formation assays, and decrease mitochondrial membrane potential (ΔΨm) at a dose-dependent manner through flow cytometry. Flow cytometry assays also revealed G0/G1 phase arrest and apoptosis in GBC-SD and NOZ cells. Western blot analysis of Sch B-treated cells revealed the upregulation of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP and downregulation of Bcl-2, NF-κB, cyclin D1 and CDK-4. Moreover, this drug also inhibited the tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data demonstrated that Sch B induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that Sch B may be a promising drug for the treatment of gallbladder cancer.

【 授权许可】

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.