Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase

Molecules

Hong Mei Li²  Seung Hwan Hwang¹  Beom Goo Kang¹  Jae Seung Hong³ 

[1] Department of Food and Nutrition, Hallym University, 200-702 Chuncheon, Korea; E-Mails:;Department of Pharmacology, College of Medicine, Hallym University, 200-702 Chuncheon, Korea; E-Mail:;Department of Physical Education, Hallym University, 200-702 Chuncheon, Korea; E-Mail:

关键词: Colocasia esculenta (L.) Schott;    aldose reductase;    antioxidant;    diabetic complication;   

DOI  :  10.3390/molecules190913212

来源: mdpi

【 摘 要 】

The goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH₂Cl₂), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H₂O) layers, using dl-glyceraldehyde as a substrate. Ten compounds, namely tryptophan (1), orientin (2), isoorientin (3), vitexin (4), isovitexin (5), luteolin-7-O-glucoside (6), luteolin-7-O-rutinoside (7), rosmarinic acid (8), 1-O-feruloyl-d-glucoside (9) and 1-O-caffeoyl-d-glucoside (10) were isolated from the EtOAc and BuOH fractions of C. esculenta. The structures of compounds 1–10 were elucidated by spectroscopic methods and comparison with previous reports. All the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity against rat lens aldose reductase. Among tested compounds, compounds 2 and 3 significantly inhibited rat lens aldose reductase, with IC₅₀ values of 1.65 and 1.92 μM, respectively. Notably, the inhibitory activity of orientin was 3.9 times greater than that of the positive control, quercetin (4.12 μM). However, the isolated compounds showed only moderate ABTS⁺ [2,29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] activity. These results suggest that flavonoid derivatives from Colocasia esculenta (L.) Schott represent potential compounds for the prevention and/or treatment of diabetic complications.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.