Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy

Molecules

Ilko Bald¹ 

[1]Institute of Chemistry—Physical Chemistry, Universität Potsdam, Karl-Liebknecht-Straße 24-25, D-14476 Potsdam, Germany

关键词: DNA origami;    atomic force microscopy;    single-molecule analysis;    DNA radiation damage;    protein binding;    enzyme reactions;    G quadruplexes;   

DOI  :  10.3390/molecules190913803

来源: mdpi

【 摘 要 】

DNA origami nanostructures allow for the arrangement of different functionalities such as proteins, specific DNA structures, nanoparticles, and various chemical modifications with unprecedented precision. The arranged functional entities can be visualized by atomic force microscopy (AFM) which enables the study of molecular processes at a single-molecular level. Examples comprise the investigation of chemical reactions, electron-induced bond breaking, enzymatic binding and cleavage events, and conformational transitions in DNA. In this paper, we provide an overview of the advances achieved in the field of single-molecule investigations by applying atomic force microscopy to functionalized DNA origami substrates.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.