Protein Expression Analyses at the Single Cell Level

Molecules

Masae Ohno¹  Peter Karagiannis¹ 

[1] id="af1-molecules-19-13932">Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center, RIKEN Address, 6-2-3 Furuedai, Suita, Osaka 565-0874, Jap

关键词: single cell analysis;    protein expression;    stochastic gene expression;    burst;    single molecule fluorescence microscopy;    proteome analysis;   

DOI  :  10.3390/molecules190913932

来源: mdpi

【 摘 要 】

The central dogma of molecular biology explains how genetic information is converted into its end product, proteins, which are responsible for the phenotypic state of the cell. Along with the protein type, the phenotypic state depends on the protein copy number. Therefore, quantification of the protein expression in a single cell is critical for quantitative characterization of the phenotypic states. Protein expression is typically a dynamic and stochastic phenomenon that cannot be well described by standard experimental methods. As an alternative, fluorescence imaging is being explored for the study of protein expression, because of its high sensitivity and high throughput. Here we review key recent progresses in fluorescence imaging-based methods and discuss their application to proteome analysis at the single cell level.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.