Study of Leaf Metabolome Modifications Induced by UV-C Radiations in Representative Vitis, Cissus and Cannabis Species by LC-MS Based Metabolomics and Antioxidant Assays

Molecules

Guillaume Marti²  Sylvain Schnee¹  Yannis Andrey²  Claudia Simoes-Pires²  Pierre-Alain Carrupt²  Jean-Luc Wolfender² 

[1] Station de recherche Agroscope, Institut des Sciences en Production Végétale IPV, Route de Duiller 50, P.O. Box 1012, Nyon 1260, Switzerland; E-Mails:;School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland; E-Mails:

关键词: phytoalexins;    UV-C stress;    metabolomics;    LC-MS;    Cannabis sativa;    Cissus antarctica;    Vitis vinifera;    chemodiversity;   

DOI  :  10.3390/molecules190914004

来源: mdpi

【 摘 要 】

UV-C radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. To assess these modifications from a global and untargeted perspective, the effects of the UV-C radiation of the leaves of three different model plant species, Cissus antarctica Vent. (Vitaceae), Vitis vinifera L. (Vitaceae) and Cannabis sativa L. (Cannabaceae), were evaluated by an LC-HRMS-based metabolomic approach. The approach enabled the detection of significant metabolite modifications in the three species studied. For all species, clear modifications of phenylpropanoid metabolism were detected that led to an increased level of stilbene derivatives. Interestingly, resveratrol and piceid levels were strongly induced by the UV-C treatment of C. antarctica leaves. In contrast, both flavonoids and stilbene polymers were upregulated in UV-C-treated Vitis leaves. In Cannabis, important changes in cinnamic acid amides and stilbene-related compounds were also detected. Overall, our results highlighted phytoalexin induction upon UV-C radiation. To evaluate whether UV-C stress radiation could enhance the biosynthesis of bioactive compounds, the antioxidant activity of extracts from control and UV-C-treated leaves was measured. The results showed increased antioxidant activity in UV-C-treated V. vinifera extracts.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.