Supramolecular Structure and Renaturation of a (1→3)-β-d-Glucan Compared with Curdlan and Scleroglucan

Fibers

Ana Isabel Puertas¹  Mª Teresa Due༚s¹ 

[1] Department of Applied Chemistry, University of the Basque Country, UPV/EHU, Pº Manuel Lardiazabal, 3, 20018 Donostia-San Sebastián, Spain; E-Mails:

关键词: (1→3)-β-d-Glucans;    curdlan;    scleroglucan;    atomic force microscope;   

DOI  :  10.3390/fib2030255

来源: mdpi

【 摘 要 】

A (1→3)-β-d-Glucan produced by Lactobacillus suebicus CUPV221 strain was investigated by tapping mode atomic force microscopy (TM-AFM), to compare its supramolecular structure and conformation with two commercial polysaccharides: curdlan and scleroglucan. It was found that the β-d-Glucan was a (1→3)(1→2)-β-d-Glucan and at room temperature formed three-dimensional networks by entanglements between strands, as does scleroglucan. However, (1→3)(1→2)-β-d-Glucan strands seemed to be more stiff than those of scleroglucan. It was also observed that curdlan samples deposited from 5 mM NaOH aqueous solution showed supermolecular assemblies, recognized in the literature as micelles, which are controlled by hydrophobic hydration. The (1→3)(1→2)-β-d-Glucan in alkaline aqueous solutions produced different supramolecular structures depending on pH, and at 0.4 M NaOH (pH 13.16), denaturation took place. After neutralizing the alkaline solution with HCl, the formation of short linear, circular, and hairpin structures was observed.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.