Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines

Toxins

Ottó Bencsik²  Tamás Papp²  Máté Berta²  Annamária Zana³  Péter Forgó³  György Dombi³  Maria A. Andersson¹  Mirja Salkinoja-Salonen¹  Csaba Vágvölgyi² 

[1] Department of Food and Environmental Sciences, Viikinkaari 9, University of Helsinki, Agnes Sjöbergin katu 2, Helsinki FI-00014, Finland; E-Mails:;Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary; E-Mails:;Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged H-6720, Hungary; E-Mails:

关键词: ophiobolin A;    boar sperm assay;    Bipolaris oryzae;    cell toxicity;    mitochondrial dissipation;   

DOI  :  10.3390/toxins6092857

来源: mdpi

【 摘 要 】

Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1–2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.