Understanding the Interaction Determinants of CAPN1 Inhibition by CAST4 from Bovines Using Molecular Modeling Techniques

Molecules

Han-Ha Chai¹  Dajeong Lim¹  Eunkyoung Jung²  Bong-Hwan Choi¹ 

[1] Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea;Insilicotech Co. Ltd., C-602 Korea Bio Park, 694-1 Sampyeong-Dong, Bundang-Gu, Seongnam-Shi 463-400, Gyeonggi-Do, Korea

关键词: CAPN1 (μ-calpain);    CAST4 (the fourth repetitive calpain-inhibition domain of calpastatin);    Bovine CAPN1/CAST4 system;    computer-aided molecular design;    anti-BVDV drug;   

DOI  :  10.3390/molecules190914316

来源: mdpi

【 摘 要 】

HCV-induced CAPN activation and its effects on virus-infected cells in a host-immune system have been studied recently. It has been shown that the HCV-nonstructural 5A protein acts as both an inducer and a substrate for host CAPN protease; it participates in suppressing the TNF-α-induced apoptosis response and downstream IFN-induced antiviral processes. However, little is known regarding the disturbance of antiviral responses generated by bovine CAPN activation by BVDV, which is a surrogate model of HCV and is one of the most destructive diseases leading to great economic losses in cattle herds worldwide. This is also thought to be associated with the effects of either small CAPN inhibitors or the natural inhibitor CAST. They mainly bind to the binding site of CAPN substrate proteins and competitively inhibit the binding of the enzyme substrates to possibly defend against the two viruses (HCV and BVDV) for anti-viral immunity. To devise a new stratagem to discover lead candidates for an anti-BVDV drug, we first attempted to understand the bovine CAPN-CAST interaction sites and the interaction constraints of local binding architectures, were well reflected in the geometry between the pharmacophore features and its shape constraints identified using our modeled bovine CAPN1/CAST4 complex structures. We propose a computer-aided molecular design of an anti-BVDV drug as a mimetic CAST inhibitor to develop a rule-based screening function for adjusting the puzzle of relationship between bovine CAPN1 and the BVDV nonstructural proteins from all of the data obtained in the study.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.