Prophylactic Protective Effects and Its Potential Mechanisms of IcarisideII on Streptozotocin Induced Spermatogenic Dysfunction

International Journal of Molecular Sciences

Yongde Xu²  Hongen Lei²  Ruili Guan²  Zhezhu Gao²  Huixi Li²  Lin Wang²  Yu Hui¹  Feng Zhou¹ 

[1] Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China; E-Mails:;Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China; E-Mails:

关键词: streptozotocin;    spermatogenic dysfunction;    IcarisideII;    oxidative stress;    diabetes mellitus;   

DOI  :  10.3390/ijms150916100

来源: mdpi

【 摘 要 】

The aim of this study was to investigate the effects of IcarisideII(ICAII) on the prevention of streptozotocin (STZ) induced spermatogenic dysfunction. Forty male Sprague-Dawley rats received intraperitoneal injection of STZ (55 mg/kg) and were equally randomized to gavage feeding of vehicle (the vehicle group) or ICAII (0.5, 1.5 or 4.5 mg/kg/day, respectively). Ten normal rats received vehicle and served as control. Four weeks later, sperm parameters, histopathological changes, testicular lipid peroxidation, antioxidant enzyme activities, and apoptosis index (AI) were evaluated. Results showed that ICAII treatment resulted in a significant recovery of sperm parameters and histopathological changes relative to the vehicle group (p < 0.05). In the vehicle group, antioxidant enzyme activities and the expression of Sertoli cell Vimentin filaments obviously decreased, while lipid peroxidation and AI significantly increased as compared with the control group (p < 0.05). Following ICAII treatment, corrective effects on these items towards normal levels were observed. The results suggested that ICAII has beneficial effect on the preservation of spermatogenic function in the STZ-induced diabetic rats. The mechanisms might be related to its improvement of antioxidant enzyme activities, preservation of the protein expression and apical extensions of Vimentin filaments, and anti-apoptosis capability.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.