The Adenovirus Genome Contributes to the Structural Stability of the Virion

Viruses

Bratati Saha¹  Carmen M. Wong¹ 

[1] Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, Canada; E-Mails:

关键词: Adenovirus;    virion stability;    viral vectors;    helper-dependent Ad;   

DOI  :  10.3390/v6093563

来源: mdpi

【 摘 要 】

Adenovirus (Ad) vectors are currently the most commonly used platform for therapeutic gene delivery in human gene therapy clinical trials. Although these vectors are effective, many researchers seek to further improve the safety and efficacy of Ad-based vectors through detailed characterization of basic Ad biology relevant to its function as a vector system. Most Ad vectors are deleted of key, or all, viral protein coding sequences, which functions to not only prevent virus replication but also increase the cloning capacity of the vector for foreign DNA. However, radical modifications to the genome size significantly decreases virion stability, suggesting that the virus genome plays a role in maintaining the physical stability of the Ad virion. Indeed, a similar relationship between genome size and virion stability has been noted for many viruses. This review discusses the impact of the genome size on Ad virion stability and emphasizes the need to consider this aspect of virus biology in Ad-based vector design.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.