Effect Analysis of Mineral Salt Concentrations on Nosiheptide Production by Streptomyces actuosus Z-10 Using Response Surface Methodology

Molecules

Wei Zhou³  Xiaohui Liu⁴  Pei Zhang¹  Pei Zhou²  Xunlong Shi² 

[1] CAS Key Laboratory of Synthetic Chemistry of Natural Substances, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China;School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China;Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433, China;College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai 201620, China

关键词: nosiheptide;    Streptomyces actuosus;    optimization;    Plackett-Burman design;    response surface methodology;   

DOI  :  10.3390/molecules191015507

来源: mdpi

【 摘 要 】

The objective of this study was to develop an optimal combination of mineral salts in the fermentation medium for nosiheptide (Nsh) production using statistical methodologies. A Plackett-Burman design (PBD) was used to evaluate the impacts of eight mineral salts on Nsh production. The results showed that among the no-significant factors, CaCO₃, and K₂HPO₄·3H₂O had positive effects, whereas FeSO₄·7H₂O, CuSO₄·5H₂O, and ZnSO₄·7H₂O had negative effects on Nsh production. The other three significant factors (Na₂SO₄, MnSO₄·H₂O, and MgSO₄·7H₂O) were further optimized by using a five-level three-factor central composite design (CCD). Experimental data were fitted to a quadratic polynomial model, which provided an effective way to determine the interactive effect of metal salts on Nsh production. The optimal values were determined to be 2.63, 0.21, and 3.37 g/L, respectively. The model also ensured a good fitting of scale-up Nsh batch fermentation with a maximum production of 1501 mg/L, representing a 1.56-fold increase compared to the original standard condition. All these results revealed that statistical optimization methodology had the potential to achieve comprehensive optimization in Nsh fermentation behaviors, which indicates a possibility to establish economical large-scale production of Nsh.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.