Syzyguim guineense Extracts Show Antioxidant Activities and Beneficial Activities on Oxidative Stress Induced by Ferric Chloride in the Liver Homogenate

Antioxidants

Constant Anatole Pieme³  Joseph Ngoupayo⁴  Claude Herve Khou-Kouz Nkoulou⁴  Bruno Moukette Moukette³  Borgia Legrand Njinkio Nono¹  Vicky Jocelyne Ama Moor³  Jacqueline Ze Minkande² 

[1] Department of Biochemistry, Faculty of Sciences; University of Yaounde I, P.O. Box 812 Yaounde, Cameroon; E-Mail:;Department of Anesthesia and Reanimation, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon; E-Mail:;Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon; E-Mails:;Department of pharmacy, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon; E-Mails:

关键词: antioxidant activity;    phenolic content;    Syzygium guineense;    radical scavenging activity;    lipid peroxidation;   

DOI  :  10.3390/antiox3030618

来源: mdpi

【 摘 要 】

The aim of this study was to determine the in vitro antioxidant activity, free radical scavenging property and the beneficial effects of extracts of various parts of Syzygium guineense in reducing oxidative stress damage in the liver. The effects of extracts on free radicals were determined on radicals DPPH, ABTS, NO and OH followed by the antioxidant properties using Ferric Reducing Antioxidant Power assay (FRAP) and hosphomolybdenum (PPMB). The phytochemical screening of these extracts was performed by determination of the phenolic content. The oxidative damage inhibition in the liver was determined by measuring malondialdehyde (MDA) as well as the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase. Overall, the bark extract of the ethanol/water or methanol showed the highest radical scavenging activities against DPPH, ABTS and OH radicals compared to the other extracts. This extract also contained the highest phenolic content implying the potential contribution of phenolic compounds towards the antioxidant activities. However, the methanol extract of the root demonstrated the highest protective effects of SOD and CAT against ferric chloride while the hydro-ethanol extract of the leaves exhibited the highest inhibitory effects on lipid peroxidation. These findings suggest that antioxidant properties of S. guineense extracts could be attributed to phenolic compounds revealed by phytochemical studies. Thus, the present results indicate clearly that the extracts of S. guineense possess antioxidant properties and could serve as free radical inhibitors or scavengers, acting possibly as primary antioxidants. The antioxidant properties of the bark extract may thus sustain its various biological activities.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.