Metabolism of Cryptic Peptides Derived from Neuropeptide FF Precursors: The Involvement of Insulin-Degrading Enzyme

International Journal of Molecular Sciences

Giuseppe Grasso²  Przemyslaw Mielczarek¹  Magdalena Niedziolka¹ 

[1] Department of Biochemistry and Neurobiology, AGH University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland; E-Mails:;Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy

关键词: IDE;    neuropeptide;    metalloprotease;    cryptome;    mass spectrometry;    surface plasmon resonance;   

DOI  :  10.3390/ijms150916787

来源: mdpi

【 摘 要 】

The term “cryptome” refers to the subset of cryptic peptides with bioactivities that are often unpredictable and very different from the parent protein. These cryptic peptides are generated by proteolytic cleavage of proteases, whose identification in vivo can be very challenging. In this work, we show that insulin-degrading enzyme (IDE) is able to degrade specific amino acid sequences present in the neuropeptide pro-NPFF_A (NPFF precursor), generating some cryptic peptides that are also observed after incubation with rat brain cortex homogenate. The reported experimental findings support the increasingly accredited hypothesis, according to which, due to its wide substrate selectivity, IDE is involved in a wide variety of physiopathological processes.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.