Polymers | |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells | |
Tiago Volkmer2  Joana Magalhs1  Vania Sousa2  Luis A. Santos2  Elena F. Burguera1  Francisco J. Blanco3  Julio San Román4  Luis M. Rodríguez-Lorenzo4  | |
[1] Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28006 Madrid, Spain; E-Mails:;Biomaterials Laboratory, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Av., 91509-900 Porto Alegre, RS, Brazil; E-Mails:;Tissue Engineering and Cellular Therapy Group (CBTTC-CHUAC), Servício de Reumatología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), C/As Xubias, 15006 A Coruña, Spain; E-Mail:;Department Polymeric Nanomaterials and Biomaterials, Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), C/Juan de la Cierva, 3, 28006 Madrid, Spain; E-Mails: | |
关键词: cryogels; α-tricalcium phosphate; PDMAEMA (Poly(dimethyl aminoethyl methacrylate)); PHEMA (poly(hydroxyethyl methacrylate); mesenchymal stem cells; | |
DOI : 10.3390/polym6102510 | |
来源: mdpi | |
【 摘 要 】
The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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