A Comprehensive Mixture of Tobacco Smoke Components Retards Orthodontic Tooth Movement via the Inhibition of Osteoclastogenesis in a Rat Model

International Journal of Molecular Sciences

Maya Nagaie³  Aki Nishiura³  Yoshitomo Honda¹  Shin-Ichi Fujiwara²  Naoyuki Matsumoto³ 

[1] Institute of Dental Research, Osaka Dental University, 8-1, Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan;Department of Chemistry, Osaka Dental University, 8-1, Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan; E-Mail:;Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1, Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan; E-Mails:

关键词: tobacco;    bone;    osteoclast;    tooth movement;    orthodontics;    nicotine;   

DOI  :  10.3390/ijms151018610

来源: mdpi

【 摘 要 】

Tobacco smoke is a complex mixture of numerous components. Nevertheless, most experiments have examined the effects of individual chemicals in tobacco smoke. The comprehensive effects of components on tooth movement and bone resorption remain unexplored. Here, we have shown that a comprehensive mixture of tobacco smoke components (TSCs) attenuated bone resorption through osteoclastogenesis inhibition, thereby retarding experimental tooth movement in a rat model. An elastic power chain (PC) inserted between the first and second maxillary molars robustly yielded experimental tooth movement within 10 days. TSC administration effectively retarded tooth movement since day 4. Histological evaluation disclosed that tooth movement induced bone resorption at two sites: in the bone marrow and the peripheral bone near the root. TSC administration significantly reduced the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclastic cells in the bone marrow cavity of the PC-treated dentition. An in vitro study indicated that the inhibitory effects of TSCs on osteoclastogenesis seemed directed more toward preosteoclasts than osteoblasts. These results indicate that the comprehensive mixture of TSCs might be a useful tool for detailed verification of the adverse effects of tobacco smoke, possibly contributing to the development of reliable treatments in various fields associated with bone resorption.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.