Sensors | |
Nanomechanical DNA Origami pH Sensors | |
Akinori Kuzuya1  Ryosuke Watanabe1  Yusei Yamanaka1  Takuya Tamaki1  Masafumi Kaino1  | |
[1] Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan; E-Mails: | |
关键词: DNA Origami; DNA Nanotechnology; Proton Detection; pH Sensors; i-motif; AFM; nanomechanical devices; Single-Molecule Sensors; | |
DOI : 10.3390/s141019329 | |
来源: mdpi | |
【 摘 要 】
Single-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like shape (DNA Origami Pliers), which consist of two levers of 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. DNA Origami Pliers can be observed as in three distinct forms; cross, antiparallel and parallel forms, and cross form is the dominant species when no additional interaction is introduced to DNA Origami Pliers. Introduction of nine pairs of 12-mer sequence (5′-AACCCCAACCCC-3′), which dimerize into i-motif quadruplexes upon protonation of cytosine, drives transition of DNA Origami Pliers from open cross form into closed parallel form under acidic conditions. Such pH-dependent transition was clearly imaged on mica in molecular resolution by AFM, showing potential application of the system to single-molecular pH sensors.
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190020851ZK.pdf | 1755KB | download |