DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin

Microarrays

Takuya Matsumoto²  Holger Feroudj²  Ryosuke Kikuchi²  Yuriko Kawana³  Hidehiro Kondo³  Ikuo Hirono³  Toshiaki Mochizuki¹  Yuji Nagashima⁴  Gen Kaneko²  Hideki Ushio²  Masaaki Kodama²  Shugo Watabe² 

[1] Kawaku Company Limited, Shimonoseki, Yamaguchi 750-0093, Japan; E-Mail:;Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan; E-Mails:;Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan; E-Mails:;Department of Food Science and Technology, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan; E-Mail:

关键词: tetrodotoxin;    pufferfish Takifugu rubripes;    microarray analysis;    gene expression;    intramuscular administration;    accumulation;    toxification;   

DOI  :  10.3390/microarrays3040226

来源: mdpi

【 摘 要 】

Pufferfish accumulate tetrodotoxin (TTX) mainly in the liver and ovary. This study aims at investigating the effect of TTX accumulation in the liver of cultured specimens of torafugu Takifugu rubripes on the hepatic gene expression by microarray analysis on Day 5 after the intramuscular administration of 0.25 mg TTX/kg body weight into the caudal muscle. TTX was detected in the liver, skin and ovary in the TTX-administered individuals. The total amount of TTX accumulated in the body was 67 ± 8% of the administered dose on Day 5. Compared with the buffer-administered control group, a total of 59 genes were significantly upregulated more than two-fold in the TTX-administered group, including those encoding chymotrypsin-like elastase family member 2A, transmembrane protein 168 and Rho GTP-activating protein 29. In contrast, a total of 427 genes were downregulated by TTX administration, including those encoding elongation factor G2, R-spondin-3, nuclear receptor activator 2 and fatty acyl-CoA hydrolase precursor. In conclusion, our results demonstrate that the intramuscular administration of TTX changes the expression of hepatic genes involved in various signaling pathways.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.