Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats

Molecules

Hélida M. L. Maranhão¹  Carlos F. B. Vasconcelos¹  Larissa A. Rolim¹  Pedro J. Rolim Neto¹  Jacinto da C. Silva Neto²  Reginaldo C. daSilva Filho⁴  Mariana P. Fernandes⁴  João H. Costa-Silva⁴  Alice V. Araújo³  Almir G. Wanderley¹ 

[1] Department of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, Brazil;Department of Histology and Embriology, Universidade Federal de Pernambuco, Recife 50670-901, Brazil;Department of Nutrition, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, Brazil;Department of Physical Education and Sport Science, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, Brazil

关键词: Simaroubaceae;    Simarouba amara;    carbon tetrachloride;    catechin;    hepatoprotection;    antioxidant;   

DOI  :  10.3390/molecules191117735

来源: mdpi

【 摘 要 】

Simarouba amara stem bark decoction has been traditionally used in Brazil to treat malaria, inflammation, fever, abdominal pain, diarrhea, wounds and as a tonic. In this study, we investigate the hepatoprotective effects of the aqueous extract of S. amara stem bark (SAAE) on CCl₄-induced hepatic damage in rats. SAAE was evaluated by high performance liquid chromatography. The animals were divided into six groups (n = 6/group). Groups I (vehicle—corn oil), II (control-CCl₄), III, IV, V and VI were pretreated during 10 consecutive days, once a day p.o, with Legalon^® 50 mg/kg b.w, SAAE at doses 100, 250 and 500 mg/kg b.w, respectively. The hepatotoxicity was induced on 11th day with 2 mL/kg of 20% CCl₄ solution. 24 h after injury, the blood samples were collected and their livers were removed to biochemical and immunohistochemical analyzes. The SAAE decreased the levels of liver markers and lipid peroxidation in all doses and increased the catalase levels at doses 250 and 500 mg/kg. Immunohistochemical results suggested hepatocyte proliferation in all doses. These results may be related to catechins present in SAAE. Thus, SAAE prevented the oxidative damage at the same time that increased regenerative and reparative capacities of the liver.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.