Candidate Gene Identification for a Lethal Chlorophyll-Deficient Mutant in Soybean

Agronomy

Sam Reed²  Taylor Atkinson²  Carly Gorecki²  Katherine Espinosa¹  Sarah Przybylski²  Alcira Susana Goggi¹  Reid G. Palmer¹  Devinder Sandhu² 

[1] Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA; E-Mails:;Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA; E-Mails:

关键词: lethal-yellow;    chlorophyll-deficient;    genetic map;    soybean;   

DOI  :  10.3390/agronomy4040462

来源: mdpi

【 摘 要 】

Chlorophyll-deficient mutants have been studied persistently to understand genetic mechanisms controlling metabolic pathways. A spontaneous chlorophyll-deficient lethal mutant was observed in self-pollinated progeny of a soybean cultivar “BSR 101”. Observed segregation patterns indicated single-gene recessive inheritance for this lethal-yellow mutant. The objectives of this investigation were to develop a genetic linkage map of the region containing the lethal-yellow (YL_PR350) gene and identify putative candidate genes for this locus. The YL_PR350 gene was mapped to chromosome 15 and is flanked by BARCSOYSSR_15_1591 and BARCSOYSSR_15_1597. This region physically spans ~153 kb and there are 14 predicted genes that lie in this region. The predicted gene Glyma.15g275900 is an excellent candidate for the YL_PR350 gene as it is homologous to an Arabidopsis gene, At3g08010, which codes for a chloroplast-localized protein (ATAB2) involved in the biogenesis of Photosystem I and II. This thylakoid membrane protein is crucial for photosynthesis in Arabidopsis. Future characterization of the candidate gene may enhance our knowledge about photosynthesis, a complex metabolic process critical for sustainability of plants.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.