Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses

International Journal of Molecular Sciences

Yan-Xu Yin¹  Shu-Bin Wang²  Huai-Juan Xiao¹  Huai-Xia Zhang¹  Zhen Zhang¹  Hua Jing¹  Ying-Li Zhang¹  Ru-Gang Chen¹  Zhen-Hui Gong¹ 

[1] College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; E-Mails:;Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China; E-Mail:

关键词: osmotic stress;    antioxidant enzymes;    Capsicum annuum L.;    CaTIP1-1;    tobacco;   

DOI  :  10.3390/ijms151120101

来源: mdpi

【 摘 要 】

Both the gene expression and activity of water channel protein can control transmembrane water movement. We have reported the overexpression of CaTIP1-1, which caused a decrease in chilling tolerance in transgenic plants by increasing the size of the stomatal pore. CaTIP1-1 expression was strongly induced by salt and mannitol stresses in pepper (Capsicum annuum). However, its biochemical and physiological functions are still unknown in transgenic tobacco. In this study, transient expression of CaTIP1-1-GFP in tobacco suspension cells revealed that the protein was localized in the tonoplast. CaTIP1-1 overexpressed in radicle exhibited vigorous growth under high salt and mannitol treatments more than wild-type plants. The overexpression of CaTIP1-1 pepper gene in tobacco enhanced the antioxidant enzyme activities and increased transcription levels of reactive oxygen species-related gene expression under osmotic stresses. Moreover, the viability of transgenic tobacco cells was higher than the wild-type after exposure to stress. The pepper plants with silenced CaTIP1-1 in P70 decreased tolerance to salt and osmotic stresses using the detached leaf method. We concluded that the CaTIP1-1 gene plays an important role in response to osmotic stresses in tobacco.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.